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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital function in advertising and marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two additional splice variants have been described but thorough nucleotide sequence facts is not but offered.
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Blocking peptides are peptides that bind specially to your concentrate on antibody and block antibody binding. These peptide ordinarily incorporates the epitope regarded by the antibody.
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This tutorial outlines even further attainable optimization of mobile and immunoassay parameters to make certain the best possible effects are...
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This guideline outlines even further probable optimization of mobile and immunoassay parameters to be certain the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind towards the epitope over the concentrate on protein. By evaluating the staining from your blocked antibody as opposed to the antibody alone, you can see which staining is distinct.
Goal Details Cyclin E belongs into the really conserved cyclin relatives, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation designs which contribute to your temporal coordination of every mitotic celebration. Cyclin E kinds a complex with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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